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The 2 protein bands from 40 kDa to 30 kDa had been absent just after 24 h incubation at 45 °C. For the rest of the bands, different concentrations of all a few proteases retained protein bands corresponding to their primary molar masses (Determine 6a,b). The existence of bands at low molecular pounds in fish roe before hydrolysis was unforeseen. In the report by Ghelichi et al. [eight], small peaks of a relatively lower molecular excess weight selection (three–fourteen kDa) ended up noticed in defatted carp roe homogenate, possibly as a consequence of an autolytic course of action occurring in fish roe and/or perhaps the defatting method resulting in the development of small-molecular-excess weight peptides.
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The incubation time for your FRAP assay was six h. Outcomes were being described since the ferric lowering antioxidant power in µM FeSO4·7H2O equivalents for every gram of sample.
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